silicon carbide fibre mediated gene transfer in cameroon

Vector less gene transfer - SlideShare

2015/11/13· GENE TRANSFER Indirect gene transfer/ Agrobacterium mediated Direct gene transfer/ Vector less gene transfer 1. Chemical methods- PEG CaPO4 precipitation DEAE-Dextran mediated Liposome mediated 2. Physical methods- Electroporation Microinjection Gene gun 3. Iibition.

Direct Gene Transfer Methods and Types - Exam …

2019/4/24· Silicon Carbide Fibre-Mediated Transformation: The silicon carbide fibres (SCF) are about 0.3-0.6 pm in diameter and 10-100 pm in length. These fibres are capable of penetrating the cell wall and plasma merane, and thus can deliver DNA into the cells.

Transformation of Microalgae Using Silicon Carbide …

Debuchy, R. L., Purton, S., and Rochaix, J.-D. (1989) The argininosuccinate lyase gene of Chlamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG7 locus.EO J. 8, 2803–2809.

Biology Exams 4 U: 5 Steps in Recoinant DNA …

Silicon Carbide fibre mediated gene transfer Ultrasound mediated gene transfer DNA transfer via pollen b. Chemical gene transfer methods: Poly Ethylene Glycol mediated (PEG mediated), Calcium Chloride mediated DEAE dextran mediated gene transfer d.

Factors affecting the genetic engineering of plants by …

1995/1/1· For example silicon carbide-mediated transformation requires the use of cell suspensions (51), which often requin considerable time to initiate and to maintain. PEG-induced transformation (82) and the electroporatio! of protoplasts (31) necessitates an efficient protoplast-to-plant system, which may take many months tt realise, with many species remaining recalcitrant (91).

Direct Gene Transfer Methods and Types - Exam …

2019/4/24· Silicon Carbide Fibre-Mediated Transformation: The silicon carbide fibres (SCF) are about 0.3-0.6 pm in diameter and 10-100 pm in length. These fibres are capable of penetrating the cell wall and plasma merane, and thus can deliver DNA into the cells.

DNA-Delivery Methods to Produce Transgenic Plants

Silicon carbide fibers: One of the recently developed methods to delivery of DNA to plants is Silicon Carbide-Mediated Transformation (SCMT). Physical and chemical characteristics of silicon carbide fibers make them capable of puncturing the cells without killing them.

A Comparison of Methods for Direct Gene Transfer into Maize …

silicon carbide-mediated gene transfer, needlelike silicon carbide fibers, or whiskers, approximately 20 Jim in length penetrate the cell wall and plasma merane to effect DNA uptake and subsequent transformation. Finally, tissue electrophoresis employs a low

Silicon carbide fiber-mediated DNA delivery into plants …

Silicon carbide fiber-mediated DNA delivery into plants cells Author KAEPPLER, H. F; WEINING GU; SOMERS, D. DNA Gene expression Ceramic fiber Scanning electron microscopy Method Nicotiana tabacum Plasmid Silicon Carbides Vortex Zea mays

GENE TRANSFER TECHNIQUES – TheGyanShakti

Silicon carbide fiber-mediated delivery of DNA into intact plant cells was investigated. Black Mexican Sweet (BMS) maize (Zea mays) and tobacco (Nicotiana tabacum) suspension culture cells were

Conference Series LLC Ltd: International Scientific and …

Title: Cell Mediated Immune (CMI) Monitoring and Its Challenges. Euro Vaccines-2015 Birmingham, UK August 17-19, 2015 Title: Healthcare In The Digital Society Health Informatics-2015 Valencia, Spain July 27-29, 2015 Title: The Threat Of Zoonotic Diseases

Top 4 Methods of Gene Transfer (With Diagrams)

The top four methods of gene transfer are: (1) DNA Transfer in Protoplasts (2) Free DNA Transfer to Intact Tissue (3) Agrobacterium Mediated Gene Transfer Method and (4) Integration and Expression. The first transgenic plant was produced via Agrobacterium mediated modified transformation of Nicotiana tabacum protoplasts by Horsch and co-workers in 1984.

Electroporation-mediated and silicon carbide fiber …

1991/1/1· SiC fiber-mediated DNA delivery The result of DNA delivery into intact cells by the silicon carbide fiber-mediated method is shown in Table I. The GUS expression was observed only when suspension cells were treated with plasmid DNA and silicon carbide fibers, whereas no GUS expression was detected when cells were treated with either plasmid DNA or fibers only, confirming the earlier …

Genotypic variation of Agrobacterium-mediated transformation …

The susceptibility of Italian ryegrass to gene manipulation has been well established by direct gene transfer methods such as particle boardment (Ye et al. 1997; Dalton et al. 1999; Takahashi et al. 2002) or by silicon-carbide fiber-mediated transformation

Semester - BPUT

Direct gene transfer methods: Particle boardment mediated transformation, Mechanism, Particle gun design, parameter for effective transformation; silicon carbide fibre mediated transformation and alternative methods.

A Review of Nanotechnology as a Novel Method of Gene Transfer …

2021/5/2· (endocytosis). This method of gene transfer has several advantages including stability of gene, reduced DNA deletion, controlled release pattern, transformability of wide range of cells and non-toxicity (Rafsanjani et al., 2012). Silicon carbide mediated

Silicon carbide fiber-mediated stable transformation of plant cells

Theor Appl Genet (1992) 84:560-566 9 Springer-Verlag 1992 Silicon carbide fiber-mediated stable transformation of plant cells * H. E Kaeppler 1, D.A. Somers 1, H.W. Rines 2, and A. E Cockburn 3

Gene Transfer to Plants - GBV

Contents Part I Agrobacterium tumefaciens-Mediated Gene Transfer 1 Introduction I Agrobacterium tumefaciens, a Natural Vector System P.J.J. Hooykaas 3 Protocols 1 Transformation of Arabidopsis thaliana Using Root Explants D. Valvekens, A. Van Gysel, M

Physical Methods of Gene Transfer | Genetics

Silicon Carbide Fiber (SCF) Mediated Transfer: SCF does not require any specialized equipment. In this approach, silicon carbide fibres in average of 0.4-0.6 µm in diameter and 10-90 µm long are taken along with DNA in vortex tube. Plant cells or eryos are

Plant transformation methods - SlideShare

2013/6/3· Silicon carbide fibre mediated 0.6 micro m dia, 10 – 80 micro m lth. vortexing in an eppendorf tube a mixture of plasmid DNA encodinga selectable or screen able marker gene, si fibre and the explantstissue. DNA delivery in his system is presumably due

Molecular Improvement of Perennial Ryegrass by …

or silicon carbide fibre-mediated gene transfer into suspension cells (Dalton et al., 1998) and after direct gene transfer into cell suspension derived protoplast (Wang et al., 1997). The time required from excision of the explants to transfer of the This long

Plant Genetic Engineering and GM Crops: Merits and …

In this paper, we present the development of a gene transfer system using DNA iibition [DI]; and/or silicone carbide fibers [SCF] treatments for gene delivery and seed-derived eryos as gene

Silicon carbide fiber mediated gene transfer – …

2021/5/9· SILICON CARBIDE FIBER MEDIATED GENE TRANSFER. The silicon carbide fibers (SCF) are about 0.3-06 µm in diameter and 10-100 µm in length. These fibers can penetrate the cell wall and plasma merane and thus can deliver DNA into the cell. The DNA coated silicon carbide fibers are vortexed with plant cells.

Methods of Gene Transfer in Plants - Biotech Articles

2013/2/23· DIRECT GENE TRANSFER (i) Pollen mediated, (ii) Iibition (iii) Microinjection (iv) Particle boardments or biolistics, (v) Electroporation, (vi) ultrasound-induced DNA uptake (vii) silicon carbide fibre -mediated DNA uptake, (viii)laser-mediated DNA uptake,

Silicon carbide fiber-mediated stable transformation of …

1992/8/1· Maize (Zea mays, cv ‘Black Mexican Sweet’) (BMS) and tobacco (Nicotiana tabacum, cv ‘Xanthi’) tissue cultures were transformed using silicon carbide fibers to deliver DNA into suspension culture cells. DNA delivery was mediated by vortexing cells in the presence of silicon carbide fibers and plasmid DNA. Maize cells were treated with a plasmid carrying both the BAR gene, whose product

Biotechnology Multiple Choice on Gene Transfer …

Biotechnology Multiple Choice on Gene Transfer Methods. 1. The virus mediated gene transfer using genetically modified bacteriophages is called. a) transfection. b) transduction. c) transformation. d) conjugation. 2. The ability of cells to take up DNA fragments from surrounding is called.

Plant Transformation and Direct Gene Transfer | Earth …

plants is Agrobacterium-mediated transformation. Agrobacterium is a naturally occurring pathogenic bacteria in the. soil that has the ability to transfer its DNA into a plant''s genome. Agrobacterium infection and gene transfer normally occurs at the. site of a wound in the plant, and causes a …

Electroporation-mediated and silicon carbide fiber …

1991/1/1· SiC fiber-mediated DNA delivery The result of DNA delivery into intact cells by the silicon carbide fiber-mediated method is shown in Table I. The GUS expression was observed only when suspension cells were treated with plasmid DNA and silicon carbide fibers, whereas no GUS expression was detected when cells were treated with either plasmid DNA or fibers only, confirming the earlier …

Transformation of Microalgae Using Silicon Carbide …

Debuchy, R. L., Purton, S., and Rochaix, J.-D. (1989) The argininosuccinate lyase gene of Chlamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG7 locus.EO J. 8, 2803–2809.

Physical methods for genetic plant transformation - …

2012/9/1· Silicon carbide fiber Ultrasound Shock waves 1. Introduction Owing to the need for improved plant cultivation there is an increasing interest in research of genetic transformations, i.e., transfer of deoxyribonucleic acid (DNA) molecules into plant cells